Potential Application of Native Lipases in the Resolution of (RS)-Phenylethylamine
نویسندگان
چکیده
O desempenho de duas lipases nativas (lipase de Aspergillus niger e Rhizopus oligosporus), e a influência da temperatura, doador acila (acetato de etila, acetato de vinila, acetato de iso-propenila e anidrido acético) e do meio orgânico foi avaliado na resolução da (RS)-feniletilamina (1). O efeito de anions em uma série de líquidos iônicos (LIs) baseados em derivados do imidazol [BMIm][X], onde X = BF 4 , PF 6 , SCN e Cl em n-heptano e acetato de vinila, também foi verificado com a lipase nativa de A. niger. Com esta lipase, a amida R-2b foi obtida com conversões de 6 até > 99% e valores de E de 2 até > 200, em n-heptano ou n-hexano. Os maiores valores de E obtidos, usando sistema bifásico formado por n-heptano e [BMIm][ PF 6 ] ou [BMIm][ BF 4 ] 9:1 (v/v), foram de 9 e 7, respectivamente, quando comparado com o solvente puro (E = 2). A influência dos ânions segue a seguinte ordem: PF 6 – >BF 4 – > SCN > Cl.
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